Creating a monstrose Pereskiopsis through grafting

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Shane
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Creating a monstrose Pereskiopsis through grafting

Post by Shane »

Inspired by these topics, I decided to try to create a monstrose Pereskiopsis. For those not wanting to read the entirety of those threads, here's a summary:
Austrocylindropuntia sublada f. monstrose (ASM) is thought to be infected with a virus which causes its monstrose growth form. Theoretically you could transmit the virus to other cacti via grafting. The newly infected cactus might take on a monstrose form. The results of others' experiments were:
Monstrose growth was induced in various opuntiads (about five results)
Degrafted Pereskiopsis continued monstrose growth (one result)
Degrafted O. compressa did not (one result)

Today I grafted an ASM offset to the bottom of a Pereskiopsis (spathulata) nub. After the graft fuses, I'll graft the ASM end to a Pereskiopsis stock (to induce growth). Here's a picture of the result. Not much to look at yet...

I will post updates as I make progress. Stay tuned
Attachments
pereskiopsis-austrocylindropuntia1.jpg
pereskiopsis-austrocylindropuntia1.jpg (61.82 KiB) Viewed 6767 times
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Pereskiopsisdotcom
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Pereskiopsisdotcom »

This is all news to me. I was not aware Austrocylindropuntia sublada f. monstrose is considered infected with a virus. Would that be any and all examples found for sale, assuming they're all coming from the same parent plant? I'll be following closely to see your results.
http://pereskiopsis.com

Interests include: Rhipsalis, Turbinicarpus, Gymnocalycium, and Lophophora.
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Shane
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Shane »

Would that be any and all examples found for sale, assuming they're all coming from the same parent plant?
I believe so. And reversion in ASM is probably from the plant suppressing the virus
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Shane
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Shane »

I unwrapped the graft today, to graft it onto another Pereskiopsis (the plan was Pereskiopsis on ASM on Pereskiopsis). The graft fused but I didn't like how the scion looked, so I decided to try again

This time I started with a bigger ASM offset
Parent plant with selected offest
Parent plant with selected offest
20200127_223514-756x1008.jpg (112.19 KiB) Viewed 6570 times
Getting started
Getting started
20200127_214959-1008x756.jpg (77.14 KiB) Viewed 6570 times
I decided to make it into two grafts. The first was pretty straightforward. I just flat grafted the Pereskiopsis nub (same one as last time) to the top of the ASM offset and wrapped it in parafilm
Wrapped graft
Wrapped graft
20200127_224339-756x643.jpg (58.71 KiB) Viewed 6570 times
I think I'm going to root this after it fuses instead of trying to graft it again. I'm not 100% decided though

I wanted to flat graft the other piece of the offset (I cut it at the bend) between two Pereakiopsis in one step. I decided this would be too difficult. I instead sliced vertically near the base of the Pereskiopsis and inserted an ASM wedge. Then I wrapped it liberally in parafilm
The insertion
The insertion
20200127_222329-756x1008.jpg (90.02 KiB) Viewed 6570 times
I hope it slid all the way in, but I can't tell theough the p-film. I'm hoping the plants exchange enough sap that the infection spreads to the Pereskiopsis
Completed graft
Completed graft
20200127_223021-756x1008.jpg (88.9 KiB) Viewed 6570 times
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Edwindwianto
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Edwindwianto »

Wow...mad scientist project
I stay tune, since i'am mad too

Keep up the creativity 👍
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Shane
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Shane »

So far the results aren't encouraging. The graft of the cuttings didn't really fuse, and I'm not sure that the insertion fused either

My mistake with the cuttings graft (in the "wrapped graft" picture) was covering the bottom of the stock. I had to unwrap to root it and I think that disturbed the graft too much. I'll cover it over the top this time and just plant it without taking the parafilm off
Los Angeles, California (USA)
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Fishhook cacti are like cats, they only like to be petted in one direction
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Shane
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Shane »

So the result of the insertion was a failure. The inserted asm tissue appears to have fused, then died. Perhaps the virus was transmitted, but I don't see any signs of it

Austrocylindropuntia just seems to be a very hard stock to graft to (at least for me). Previously I did a bunch of experiments grafting to normal A. subulata and though some fused, none survived more than ~10 days (they looked fine for a while then dried up). I tried different amounts of pressure and humidity to no avail
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Edwindwianto
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Edwindwianto »

Good morning Shane

What has become of this experiment?
You stop experimenting?

I'm curious to know if there is actually virus in ASM

EDWIN
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Shane
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Shane »

Edwindwianto wrote: Mon Mar 23, 2020 1:09 am Good morning Shane

What has become of this experiment?
You stop experimenting?

I'm curious to know if there is actually virus in ASM

EDWIN
I decided to use a larger piece of ASM. I currently have two cuttings a neighbor gave me. They're still in the process of rooting, but when they're rooted I plan to graft Pereskiopsis on top of one. I haven't checked the Pereskiopsis I did the tissue insertion on in a while. I doubt it's gone monstrose, but I'll check tomorrow

I also have a piece of Opuntia monacantha monstrose variegated I plan to do a similar experiment with. I've heard it has a virus as well
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Edwindwianto »

Shane wrote: Mon Mar 23, 2020 3:36 am I decided to use a larger piece of ASM. I currently have two cuttings a neighbor gave me. They're still in the process of rooting, but when they're rooted I plan to graft Pereskiopsis on top of one. I haven't checked the Pereskiopsis I did the tissue insertion on in a while. I doubt it's gone monstrose, but I'll check tomorrow

I also have a piece of Opuntia monacantha monstrose variegated I plan to do a similar experiment with. I've heard it has a virus as well
Thanks Shane

I'am really interested with this virus transmission thing

I'll stay tune

EDWIN
Jitesh Rana
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Jitesh Rana »

Hey Shane,

I have graft Opuntia subulata monstrose on pereskiopsis first, then i wil try to graft something on it
The result i got after Grafting monstrose on peres are O.S.monstrose have shape changed and more spines than the natural one

I also graft S.monstrose on opuntia but it is like normal S.monstrose
Attachments
More spines
More spines
IMG_20200604_29421.jpg (50.19 KiB) Viewed 5960 times
IMG_20200604_11173.jpg
IMG_20200604_11173.jpg (47.58 KiB) Viewed 5960 times
IMG_20200604_1691.jpg
IMG_20200604_1691.jpg (48.7 KiB) Viewed 5960 times
Shape differ than that of natural
Shape differ than that of natural
IMG_20200604_2411.jpg (52.83 KiB) Viewed 5960 times
On opuntia grafted
On opuntia grafted
IMG_20200604_42491.jpg (52.84 KiB) Viewed 5960 times
I have this Opuntia sabulata monstrose Cactus
I have this Opuntia sabulata monstrose Cactus
IMG_20200604_8629.jpg (42.07 KiB) Viewed 5960 times
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Shane
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Shane »

Jitesh Rana wrote: Thu Jun 04, 2020 3:41 am I have graft Opuntia subulata monstrose on pereskiopsis first, then i wil try to graft something on it
I think that's a good strategy, as long as you don't use two tiny pieces of cactus like I did. I successfully grafted a tiny offset of O. monacantha 'Joseph's Coat' (monstrose and variegated, also thought to be caused by an infectious agent) to Pereskiopsis last year to try this. It's just now gotten big enough that I think I can do the next graft

As for the Austrocylindropuntia, it's also now ready for grafting (it had scale, which I wanted to take care of before I used it as a stock). Hopefully I can work up the courage to try it soon...

I've also been delayed by poor Pereskiopsis growth. It hasn't liked the weather here until recently (believe it hates lows below 50 or 60 F)

I'll post the results of both grafts here
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Re: Creating a monstrose Pereskiopsis through grafting

Post by anttisepp »

Shane wrote: Wed Jan 22, 2020 8:03 pm <...> reversion in ASM is probably from the plant suppressing the virus
I'd correct that not virus (it's difficult to get rid of virus infection), but Phytoplasma, and very possible to gain healty apex if plant grows relatively fast. Many so called monstros forms have strong fast growing healthy sprouts free of Phytoplasma. Some years ago I also gained healthy sprouts from monstrose Opuntia ficus indica and Austrocylindropuntia subulata. In the case of grafting infection move to grafted plant and cause monstrose growth.
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Jitesh Rana »

I am thinking of degrafting O. Sabulata monstrose to see if it retain this shape or not
So my qstn is how to degraft should i just pluck off Sabulata monstrose or cut somewhere
and
i have grafted a red spine Opuntia on sabulata monstrose on pereskiopsis 2 days ago
IMG_20200604_18915.jpg
IMG_20200604_18915.jpg (52.63 KiB) Viewed 5941 times
I will post an update after it has some growth
Attachments
Where to degraft this one
Where to degraft this one
IMG_20200604_1691.jpg (48.69 KiB) Viewed 5941 times
IMG_20200604_1380.jpg
IMG_20200604_1380.jpg (41.64 KiB) Viewed 5941 times
Jitesh Rana
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Re: Creating a monstrose Pereskiopsis through grafting

Post by Jitesh Rana »

Update on Austrocylindropuntia on pereskiopsis
Pup appears
Pup appears
IMG_20200622_130119.jpg (37.69 KiB) Viewed 5845 times
and other graft

The pereskiopsis which is grafted on {Austrocylindropuntia on pereskiopsis} seem to like have Normal growth
Not monstrose growth till now
Not monstrose growth till now
IMG_20200622_125810.jpg (41.8 KiB) Viewed 5845 times
for now , idk may be later it have monstrose growth

And another graft red spines opuntia on peres on Austrocylindropuntia on peres. Does not have growth
IMG_20200622_125024.jpg
IMG_20200622_125024.jpg (51.89 KiB) Viewed 5845 times
maybe merging failed or Very small part merge
Attachments
Pup appear
Pup appear
IMG_20200622_125904.jpg (29.91 KiB) Viewed 5845 times
Another peres on Austrocylindropuntia on peres
Another peres on Austrocylindropuntia on peres
IMG_20200622_125351.jpg (46.77 KiB) Viewed 5845 times
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