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Hello, I'm a fellow grower from Costa Rica. I've been growing cacti for over 4 years now and doing a lot of micrografting.
I usually use selenicereus grandiflora and pereskiopsis, both of those grow very well in my climate.
Nevertheless, I sometimes have problems with this stuff. I haven't being able to determine what is causing this to appear in my graft unions. The scion usually grows for a couple of weeks and then gets stuck. I Always wash my hands before grafting and clean my tools with 70% alcohol and a flame. I'm thinking on using 96% etilic alcohol.

Usually, this layer stays as is, a layer.
But other times, it grows (also the scion) and makes some kind of necrotic cork.
Usually, regrafting is the solution, and it just goes away. It only happens with micrografts, I haven't seen this problem in regular grafts yet.



Any suggestions in how to avoid this issue?

when this happened to my grafted plants, I assumed that it happened because of unsanitary blade or because the scion was rotting

cacthis wrote: ↑Thu Jul 01, 2021 11:59 am when this happened to my grafted plants, I assumed that it happened because of unsanitary blade or because the scion was rotting

how do you sterilize your tools? If I may ask.

I used alcohol to wipe the knife

My gut feeling is your problem is airborne or in the soil. I've been grafting on Pereskiopsis for about a decade now and I would see that orange colour on failed grafts only when I had the humidity too high with something like a dome covering the plants and too much stale air.

There have been a number of times that I've been in a rush and forgot sterilization, never encountering that problem. There have also been times that I have sterilized like I was worried about Covid and I still got that color and failed graft.

I've been grafting with parafilm. I'm from Costa Rica, so my humidity Is always high

Looks like a fungal issue to me, with the high humidity it wouldn't be a surprise

Charlie VIII wrote: ↑Sun Jul 04, 2021 5:22 am Looks like a fungal issue to me, with the high humidity it wouldn't be a surprise

How could I reduce humidity without the scion getting dehydrated?

G'day eamnav215,

Your stock is a selenicereus.
The grafting is a micro-grafting.
A. parafilm is used for the grafting.

Please let me know the size of scion.

Frank

Hanazono wrote: ↑Wed Jul 07, 2021 12:42 am G'day eamnav215,

Your stock is a selenicereus.
The grafting is a micro-grafting.
A. parafilm is used for the grafting.

Please let me know the size of scion.

Frank

Between 3 and 5 mm,I usually graft 2 week old plants. Most problems with ariocarpus. I bought a box today, I'll try your humidity box method.