Degrafting

[Hanazono]

The drying process of degrafted Ariocarpus was completed and I set them for the rooting in this morning.
1. Cleaned drying medium up

2. filed the cavity up by wet cray grains
The grain size is ~1 mm.

3. Set degrafted scions into 10 cm square pots
Rooting medium is wet pumice sand.

[Hanazono]

It has been still mid of winter in here but I have started to prepare degrafting/rooting.
I degrafted 5 scions in this morning.

Scions: Asrophytum hybrid hakujou fukuryu banjyaku
Stocks: H. jusbertii
Since the rooting of this species is easy because of mixing ornatum gene, I used a normal degrafting method.
The grafting joint of H. jusbertii stock is not embedded and I broke the scion off at the joint.

Grafted scions

A scion broken off at the joint

Cut joint off completely

Used garden lime as drying agent and set scions onto black plastic pots
They will be kept until the end of August for drying and rooting.

[Hanazono]

Although Turbinicarpus alonsoi is a small cactus but it grows to a large one when you grafted it.
I had 7 grafted ones grafted in Feb. 2007.
I used the method of degrafting with a short permanent stock.
I degrafted all of them in this morning.

Before degrafting
The stock is a H. jusbertii, a permanent stock.

Pre-cutting
A scissors was used for the pre-cutting

Adjusting stock length
The stock was cut to 15 ~ 20 mm length and removed all spines to prevent offsets formation.

Drying
Garden lime was sprinkled over cutting faces and set it on a empty plastic pot.
The cactus will set on soil in the end of August.


I used this this method quite many times and got good results.
The photo is the one, degrafted several years ago.

[Hanazono]

7 of degrafted Echinocactus horizontharonius were set for rooting.

Degrafted in July and set for rooting on 21st August
The dried scion has a dent.

Rooting medium is pumice sand, watered before setting

The dent is filled up by wet cray grains.

The degrafted scion is set on the rooting medium.

7 scions are set for the rooting.

[Hanazono]

6 of degrafted Escobaria minimas were set for the rooting on 22nd August.

[Hanazono]

4 of degrafted Astrophytum hybrids, myriostigma x caput-medusae were set for the rooting on 23rd August.

I could get small number of seeds and sowed in 2008.
The germination rate was poor and just 4 of them germinated.
I grafted seedlings in same year.

The growing speed was very slow and I could not see any flowers.
I degrafted them last month.

I assume the hybridization affects on and appearances are not so good.
The size is around 7 cm in diameter.

[snarfie]

Maybe you should do a backcross with those to the caput-medusae?

[DaveW]

I may eventually try your method of rooting Aztekium ritteri Hanazono, leaving some of the stocks vascular bundles on. However I would sooner try to root offsets since my plant is now offsetting. Have you ever had any luck rooting these offsets since of course they have no vascular bundles from the stock to root down.

Another plant I had trouble with trying to root down from a graft is Mammillaria luethyi. It just sits there and shrivels away without rooting. In fact I sometimes have trouble with it shrivelling on the stock in winter. The stock remains perfectly healthy but the scion shrivels leaving a dried union. I wonder whether it is differential shrinkage between scion and stock that breaks the union in the resting season since the stock is still perfectly turgid and healthy?

I was thinking of asking a friend who works with tissue culture if he knows if degrafted plants could be rooted on that agar type medium they use, since it seems to be able to grow plants from small pieces of tissue and eventually form roots on them?

[Hanazono]

snerfie

Unfortunately I have not seen any flowers of those cacti yet.
I will backcross when they were in flower.

Hanazono

[Hanazono]

DaveW

I have tried but I have never gotten any successful results of rooting for A. ritteri off-sets.

I experience same problem of grafted M. luethyi.
I think the rooting of luethyi is easy normally and tap roots will grow as like seedlings.
You should review the method of your rooting.

I found the article of cacti propagation from tissue on a Japanese website.
They are selling a simple propagation kit but I have not tried it yet.

Hanazono

[DaveW]

I was wondering if some form of tissue culture variation could be used for rooting Aztekium ritteri or Mammillaria luethyi offsets, or even their degrafts on tissue culture gel to get roots started before transferring them to soil? You seem to be able to create a new rooted plant from just a small amount of tissue so why should it not put roots an a larger chunk of tissue, the degraft itself? If you scroll down the following link to the post by "Hippie" I was thinking of something similar to his petri dish method, or even using a glass jar if petri dish not high enough.

https://mycotopia.net/topic/29688-agart ... leafclone/

I was also thinking of some more simple variation of using tissue culture gel like this. Has anybody ever tried it for cacti?

https://www.jungseed.com/P/51228/Gel+2+ ... er+2+Count

[Hanazono]

Degrafted super kabutos were attacked by mealybugs.
I degrafted them with a short H. jusbertii stocks 10 years ago.
All mealybugs were washed away by running water and roots were trimmed.

The stock turns to woody structure and to be a part of the scion.
Roots have developed only from the stock.
H. jusbertii is a permanent stock.

[abdulhafezzahruddin]

Hi Hanazono...
I am trying to do this technique of degrafting...
How long should we wait before we can put this on pumice for rooting? Do we need to wait until dry too?

I went to degrafting with a short vascular bundle of Hylocereus.
The attached photo is the prepared scion before rooting.

Roots will come out from the vascular bundle initially very soon and rely on them for some years.
Roots will develop from the scion just before the vascular bundle die.

[Hanazono]

G'morning abdulhafezzahruddin,

You should not dry the vascular bundle at all.
The vascular bundle will die when it was dried.

Prepare a pot filled in pumice and water.
Prepare scion with the vascular bundle as shown on the photo.
Set the scion into the wet pumice immediately without drying.
The vascular bundle does not rot with this condition because the soft tissue of stock is removed.

Frank

[Hanazono]

2 of Ariocarpus posted photos on 8th January 2017 were in flower in this afternoon.
They also have rooted well.